Biocontrol of Aspergillus flavus Strains Isolated from Bambara Groundnut (Vigna subterranea (L.) Verdc.) Seeds Using Essential Oils of Lippia multiflora Moldenke, Ocimum americanum L. (Lamiaceae) and Eucalyptus cameldulensis Dehnh

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Résumé

In nature, plant extracts play a crucial role in defending plants against biotic and abiotic stressors. Moreover, the use of plant-based products, such as plant extracts, represents a promising alternative to synthetic fungicides, which pose potential health risks to consumers. In this study, the antifungal activity of the essential oils (EOs) of Lippia multiflora, Eucalyptus camaldulensis and Ocimum americanum was evaluated against two strains of Aspergillus flavus via the agar dilution method. These two Aspergillus flavus fungi was isolated from Bamabra groundnut seeds. Lippia multiflora essential oil (EO) showed the best results compared with the other oils, with a minimum inhibitory concentration (MIC) of 9000 μg∙mL−1. The MIC for Eucalyptus camaldulensis and Ocimum americanum EOs was 10,800 μg∙mL−1. In view of their antifungal properties, these EOs could be used to develop a new, safe antifungal agent for food preservation.

Mots-clés

Aspergillus flavus, Essential Oil, Antifungal, Bambara Groundnut