The main objective of this work was to identify chemical compounds of biological interest in the methanol extract of Acacia macrostachya stem bark using High-Performance Liquid Chromatography coupled with tandem Mass Spectrometry (HPLC-MS/MS) and Global Natural Product Social Molecular Networking (GNPS) databases. A total of seven (07) molecules from four families of secondary metabolites such as Kaempferol-3-O-rutinoside, kaempferol, epicatechin, epicatechin-3-O-gallate, bergaptol-O-β-D-glucopyranoside, 3-hydroxyurs-12-en-28-oic acid and kaempferid were identified The presence of these compounds in the extract could be responsible of the anti-diabetic and anti-cancer properties of this extract which were reported in our previous investigations.

Acacia macrostachya, HPLC-ESI-MS/MS, flavonoids, tannins, terpenoids