A screen-printed electrode (SPE) was successfully activated and modified by electrodeposition of nickel (II) tetrasulfonated phthalocyanine film (poly-NiTSPc) for the electrochemical analysis of para-aminophenol (PAP). Cyclic voltammetry, differential pulse voltammetry, electrochemical impedance spectroscopy, optical microscopy and scanning electron microscopy coupled with energy dispersive X-ray (EDX) experiments were performed to characterize the SPE. Calibration curves were determined in the concentration range of 0.1 mg l−1 to 2.4 mg l−1 using the tested electrodes and linear relationships were resolved between peak current intensities and PAP concentrations. The limits of detection (LOD) obtained were 74 μg l−1, 34 μg l−1, 29 μg l−1 and 26 μg l−1 for unmodified SPE, poly-NiTSPc/SPE, activated/SPE and poly-NiTSPc/activated SPE, respectively. The poly-NiTSPc/activated SPE was applied for the bioremediation study of PAP using Trichoderma harzianum in a fungal microbial fuel cell (MFC). Our results showed a first-order kinetic degradation with a kinetic constant of 0.063 h−1 at 20 °C and a half-time of degradation of 11 h for an initial concentration of 100 mg l−1. Subsequently, we assessed the poly-NiTSPc/activated SPE for PAP identification as a by-product of crystal violet degradation in a photocatalytic system using Ag/FeVO4 as a photocatalyzor in the presence of H2O2.

3-methyl*4-nitrophenol, nanofiltration, layer-by-layer, microbial fuel cell