Odontonema strictum (Acanthaceae) leave extract was investigated for its flavonoid content. Column chromatography was used for compound isolation and mass spectrometry was performed using electrospray ionization (ESI) in the negative ion mode for compound identification.
The full characterization of luteolin 7-O-[β-D-apiofuranosyl-(1 → 2)-O-β-D-ribofuranoside], a flavone glycoside, was achieved using tandem mass spectrometry and high resolution 1D and 2D Nuclear Magnetic Resonance (NMR). Among the 10 flavonoids glycosides detected in the ethanol extract, beside the isolated one, 3 flavone glycosides with luteolin or apigenin aglycone were tentatively identified.

Odontonema strictum (Acanthaceae), Flavonoids, LC-MS, NMR