Rice blast caused by Magnaporthe oryzae is one of the major rice diseases in Burkina Faso with losses up to 77% under favorable disease conditions. For the management of this disease, the use of resistant cultivars remains the most economical, and most protective method for the environment. This study focuses on the evaluation of the resistance of 49 lines of rice resulting from crosses between popular cultivars of different countries of Sub-Sahara against blast. The experimental design used is a 7 x 7 Alpha lattice with 3 repetitions. The study was conducted in two rainfed sites (Farako-Bâ and Karfiguela) and two irrigated sites (Bagré and Tengrela) in Burkina Faso. The results showed that the rice genotypes developed the disease differently depending on their developmental stages and rice growing systems. In rainfed rice cultivation, 32 genotypes were resistant to leaf blast and 3 (AR-67, IR 130412 and CSR 36) were resistant to leaf and panicle blast. In irrigated conditions, 44 genotypes were resistant to leaf blast and 6 (TZLR-74, IR 133136-B, NERICA 4, NERICA 10, NERICA 11 and CSR 36) were resistant to leaf and panicle blast. The genotype (CSR 36) was disease resistant in both ecological conditions. The results of this study will make it possible to choose the best rice cultivars, tolerant or resistant to blast, and to identify the effective resistance genes in their genomes.