The availability of scientific information useful for the orientation of artisanal and industrial tinctorial practicesto ensure the protection of the environment and the health of artisans and consumers remains a major concern fora large number of actors. Qualitative, quantitative analyses and structural identifications works usingspectrophotometric, chromatographic and spectral methods were carried out on the total aqueous extract fromleaves of A. leiocarpus. This extract was used to dye skeins of cotton fibers. With an extraction yield of about 5%, A. leiocarpus leaves gave a total flavonoid content of the order 458.759±27.773 mgEQ/g of dye powder.Hydrolysable and condensed tannins rates are 13.25 % and 12.96 %, respectively. The HPTLC chromatographicprofile of the dye showed that the extract of the A. leiocarpus leaves contains flavonols. The elongationvibrations νO-H and νC-O respectively of alcohols and oxide ethers in infra-Red testify to the presence of flavonic-type dyeing molecules which, by the phenomenon of co-pigmentation, contribute to the final shades in tinctorialpractice. High-performance liquid chromatography coupled with mass spectroscopy revealed the presence ofmajor flavonoid molecules such as quercetin 3-O-rhamnoside, quercetin 3-O-glucuronide and kaempferol 3-O-hexoside. Tinctorial practice techniques applied to skeins of cotton fibers have resulted in shades of variouscolours ranging from anise to chartreuse green. In addition to being a natural acid dye, the textile dye extractedfrom A. leiocarpus leaves can be classified in the group of metal dyes in terms of the quality of the shadesobtained with the use of mordants such as alum, hydrated iron and copper sulphates

(9) (PDF) HPLC-MS identification of three major flavonoids in the textile dye extract from dried leaves of Anogeissus leiocarpus. Available from: https://www.researchgate.net/publication/359156044_HPLC-MS_identification_of_three_major_flavonoids_in_the_textile_dye_extract_from_dried_leaves_of_Anogeissus_leiocarpus [accessed Feb 29 2024].

Flavonoids, Tannins, HPTLC, HPLC-MS/MS