Viral and bacterial infections represent an occupational risk for female sex workers. This
study aimed at determining HPV coinfection with genital pathogens among female sex workers
inWest and Central Africa and identifying antibiotic resistance genes. A total of 182 samples from
female sex workers were analyzed by real-time PCR and classic PCR. For the molecular diagnosis of HPV, the real-time multiplex amplification kit “HPV Genotypes 14 Real-TM Quant” from SACACE
Biotechnologies®, detecting 14 high-risk HPV genotypes, was used, while for other pathogens, the
real-time multiplex amplification kit N. gonorrhoeae/C. trachomatis/M. genitalium/T. vaginalis Real-TM, allowing their simultaneous detection, was used. The women were aged 17–50 years with an average age of 27.12 ± 6.09 years. The pathogens identified were HPV 54.94% (100/120), Neisseria gonorrhoeae (13.74%), Chlamydia trachomatis (11.54%) and Mycoplasma genitalium (11.54%). The most common HPV genotypes were HPV68, HPV38 and HPV52. The antibiotic resistance genes identified were bla QNR B 24.00%, bla GES 22.00%, bla SHV 17.00%, bla CTX-M 13.00% and bla QNR S 1.00%. This study revealed the presence of various HPV genotypes associated with other pathogens with problems of antibiotic resistance among sex workers ofWest and Central African origin working in Ouagadougou.