The evolution of increasingly antimicrobial-resistant bacterial species in general and particularly. The emergence of strains of methicillin-resistant Staphylococcus aureus (MRSA) are currently a real threat to humanity. There is an urgent need for new efficient antibiotics. Medicinal plants can be the sources of effective new therapeutic agents. This study as performed to study the antimicrobial activity of three medicinal plants (Mitragyna inermis, Vetiveria nigritana, Kalanchoe crenata) from Burkina Faso against Staphylococcus aureus strains isolate from patients. The antibiotics susceptibility of Staphylococcus aureus strains and the antimicrobial activities of the plant extracts were evaluated using standard agar disc diffusion method. Determination of minimum inhibitory concentrations
(MIC) and minimum Bactericidal Concentration (MBC) of the active extracts was done using the agar microdilution method. The highest antimicrobial activity was recorded with ethanol extracts of plants against MRSA. No antimicrobial activity was detected with decoction extracts. The MIC and MBC of the different extracts ranged from 0.625 to10 mg/ml for Mitragyna inermis and Vetiveria nigritana extracts and from 0.625 to 5 mg/ml for Kalanchoe crenata extracts. Mitragyna inermis, Vetiveria nigritana, Kalanchoe crenata are some therapeutically potential plants to combat microbial infections due to MRSA.