BACKGROUND: High-risk human papillomavirus (HPV-HR) infections are responsible
for 99.99% of cervico-uterine cancers and 50% of carcinomas of the oropharynx.
OBJECTIVE: To characterize high-risk HPV genotypes (HPV-HR) in histologically
confirmed ear, nose and throat (ENT) cancers in Ouagadougou.
METHODS: One hundred and twenty-eight archived tissues from the ENT sphere,
obtained over the last ten years (2007 to 2017) and histologically diagnosed in
anatomy and pathology-cytology laboratories in Ouagadougou were included. These
tissues were dewaxed with xylene; HPV DNA extraction was performed and HPV-HR
were researched by real-time multiplex PCR.
RESULTS: Among the fourteen HPV-HR genotypes tested for, seven were identified.
The prevalence of HPV-HR infection was 15.6%. The most common genotypes were:
HPV56 (45%) and HPV33 (20%). Squamous cell carcinomas accounted for 75% of
cases, followed by lymphomas for 10%. The age range was between 5 and 80 years.
CONCLUSION: The results show the involvement of a diversity of HPV-HR genotypes
and a high frequency of HPV56 and HPV33 in ENT cancers in Ouagadougou, Burkina
Faso. The appropriate HPV vaccination will considerably reduce the number of
these cancers

high-risk HPV; Burkina Faso; Carcinoma; ENT; Real-Time PCR