Insecticides are commonly used in greenhouses or fields to control Bemisia tabaci populations leading to rapid evolution of resistance that make treatments inefficient. This resistance can be mediated by molecular and/or biochemical mechanisms. Here, we propose to determine the detoxifying enzymes involved in B. tabaci resistance and to establish the relationship between the enzymatic activity and the values of Lethal Concentrations 50 (LC50). Susceptibility to deltamethrin, acetamiprid and chlorpyrifos-ethyl was assessed, using leaf dip method populations sampled from four (4) sites in Burkina Faso in 2017. Detoxifying enzymes activities including non-specific α-esterases, Para Nitro Phenyl Acetate esterases, oxidases (cytochrome P450) and Glutathione -S-Transferases (GSTs) were measured on single B. tabaci (N = 50) from each test locality and compared with the susceptible strain. In all sites, B. tabaci demonstrated multiple resistance phenotypes. Variation in detoxifying enzyme activities was observed within B. tabaci populations. Elevated levels of nonspecific α esterase and GSTs were widespread, suggesting multiple resistance mechanisms occurred within B. tabaci populations from this country.

Bemisia tabaci . Insecticide resistance . Detoxificant enzymes . Burkina Faso