Six varieties of mangoes (Amelia, Brooks, Kent, Keitt, Lippens, Springfield) harvested from different pedological areas of the western region of Burkina Faso (Bobo-Dioulasso, Orodara, Banfora) were analyzed by high performance liquid chromatography in order to determine the β-carotene content. The study revealed a variability in β-carotene content between different varieties and regions. The Amelia variety is the richest in β-carotene content and is a good source of provitamin A.