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Regulation by CRAMP of the responses of murine peritoneal macrophages to extracellular ATP,
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Auteur(s): E. KABRE
Auteur(s) tagués: KABRE Elie
Renseignée par : KABRE Elie
Résumé

Peritoneal macrophages were isolated from wild type (WT) mice and from mice invalidated for the P2X7 receptor (KO) which had been pretreated with thioglycolate. In cells from WT mice, 1 mM ATP increased the intracellular concentration of calcium ([Ca2+] i), the uptake of ethidium bromide, the production of reactive oxygen species (ROS), the secretion of IL-1β, the release of oleic acid and of lactate dehydrogenase; it decreased the intracellular concentration of potassium ([K+] i). In KO mice, ATP transiently increased the [Ca2+] i confirming that the P2X7 receptor is a major receptor of peritoneal macrophages. WKYMVm, an agonist of receptors for formylated peptides (FPR) also increased the [Ca2+] i in murine macrophages. The slight increase of the [Ca2+] i was strongly potentiated by ivermectin confirming the expression of functional P2X4 receptors by murine peritoneal …

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