Search for a suitable substrate for mass propagation of a local strain of Trichoderma harzianum (ThTab) isolated in Burkina Faso

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Résumé

A strain of Trichoderma harzianum Pers. isolated in Burkina Faso showed significant
antagonistic properties against phytopathogenic fungi in vitro and in the greenhouse. The aim of this study
was to identify an effective, available and inexpensive medium for its multiplication and large-scale use.
Thus, vari ous organic substrates identified as carbon sources were supplemented with others
identified as nitrogen sources and then tested using the solid-state fermentation method. An inoculum of the
fungus was fermented on these slightly humified substrates for 07 days at room temperature (22-25°C). The
number of micropropagules produced per substrate was then evaluated in Colony Forming Units per gram
of substrate (CFU/gos). The use of maize bran supplemented with soy flour as a substrate resulted in an
average micropropagule production of 1045.5.108 CFU/gos, i.e. a 5228-fold multiplication of the initial
inoculum. Maize bran was the best carbon source, with an average contribution of 297,108 CFU/gos
whatever the nitrogen supplement, and soy flour the best nitrogen supplement, with an average contribution
of 113.7,108 CFU/g0s whatever the carbon source. The development of a formulation based on maize bran
and soy flour for the mass multiplication of this strain is envisaged.

Mots-clés

Trichoderma harzianum, Micro propagules, Maize bran, Burkina Faso, Substrate