Resistance to Beta-lactams by Klebsiella Co-ProducingResistance Enzymes at the Pietro Annigoni ResearchCentre (CERBA)

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Résumé

The misuse of antibiotics promotes the development of multi-resistance in bacteria both biochemically and genetically, as well asits ability to transmit to other bacteria. These microorganisms are capable of simultaneously producing resistance enzymesthrough resistance mechanisms that allow them to resist various classes of antibiotics at the same time and thus becomemulti-resistant. Our objective was to study resistance to beta-lactams by Klebsiella co-producing resistance enzymes isolated atthe Pietro Annigoni Research Center (CERBA). The isolation and purification of bacterial strains isolated from stools, vaginalswabs and urine of internal and external patients of CERBA, were carried out on selective media and Muller Hinton (MH). Theantibiogram was carried out according to the disk diffusion method. The API 20E biochemical gallery (Bio Mérieux, France) wasused for the identification of enterobacteria and the blaNDM, blaSHV and blaTOHO genes were detected by conventional PolymeraseChain Reaction (PCR). A total of one hundred and twenty-two (122) strains of Gram-negative bacilli were collected andidentified. Among them we have 23.77% (29/122) strains of Klebsiella including 86.21% isolated from urine, 6.90% isolatedfrom stool and 6.90% isolated from vaginal swab. The antibiogram showed that all 29 Klebsiella strains were resistant to at leastone of the beta-lactams studied, including 93.10% resistance to amoxicillin plus clavulanic acid, 37.93% resistance toceftazidime, 27.59% resistance to ceftriaxone, 44.83% to cefotaxime, 20.70% to imipenem and 24.14% to aztreonam.

Mots-clés

Multi-Resistance, Antibiotic, Enterobacteria, Klebsiella, BlaNDM, BlaSHV, BlaTOHO, Co-Production