Antibacterial activity of methanol bark extract from Acacia dudgeoni craib ex holl (Mimosaceae) on growth of cefotaxime resistant Escherichia coli

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Résumé

The development of bacterial resistance to available antibiotics is a worldwide problem and recent researches focused on the development of new strategies to combat this bacterial resistance. The current study was designed to evaluate the inhibitory property of the methanol bark extract from Acacia dudgeoni on cefotaxime resistant growth of Escherichia coli. The capacity of extract to inhibit the E. coli growth was evaluated in vitro by using agar diffusion assay. The free radical scavenging property of extract was assessed by using the standard DPPH assay. Furthermore, flavonoids and phenolic contents of extract were determined spectrophotometrically. Extract at concentration of 50 μg/mL showed an interesting inhibitory activity on E. coli strain 376 growth that is resistant to the antibiotic cefotaxime. Moreover, extract exhibited a good DPPH scavenging activity with IC50value ranged from 16.52 ± 1.04 μg/mL. The flavonoid and phenolic contents of extract were 7.31 ± 0.2 mg EQ/gof extract and 14.25 ± 1.25 mg EAG / g extract respectively. The methanol bark extract from A. dudgeoni is apotent source of bioactive compounds to combat bacterial resistance.

Mots-clés

Acacia dudgeoni, cefotaximase, Bacterial resistance, Escherichia coli