FIRST DETECTION OF SHV-TYPE EXTENDED SPECTRUM Β-LACTAMASES IN THE UNIVERSITY HOSPITAL COMPLEX PAEDIATRIC CHARLES DE GAULLE (CHUP-CDG) OF OUAGADOUGOU IN BURKINA FASO
- Journal of Asian Scientific Research , 4 (5) : 214-221
Résumé
Strains were isolated from urines clinical samples from patients from various service units of University Hospital complex Paediatric Charles De Gaulle (CHUP-CDG) in Burkina Faso. These strains were resistant to at least one oxyimino-cephalosporin. They were identified as producer of extended spectrum β-lactamases (ESBL) by double-disk synergy test between amoxicillin clavulanate and cefotaxime, ceftriaxone or ceftazidime and by kinetic methods. Crude extract from these isolates showed a broad-substrate profile by hydrolyzing cefuroxime, cefotaxime, ceftriaxone and with a lower degree to cetazidime and cefepime but not imipinem. Sequencing of PCR products amplified with primers designed for blaSHV genes showed that these genes coded for SHV-11, SHV-12, SHV-28, SHV-32, SHV-38, SHV-76 and SHV-99. This is the first description of these enzymes in Burkina Faso.
Mots-clés
SHV-type extended spectrum β-lactamases, University hospital complex paediatric charles de gaulle.