Isolation and identification of Escherichia coli and Klebsiella pneumoniae strains resistant to oxyimino-cephalosporins and monobactams through the production of GES-type extended-spectrum beta-lactamases (ESBLs) at the Saint-Camille Medical Centre in Ouagadougou, Burkina Faso.

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Résumé

Background: Bacterial resistance to beta-lactams is a significant public health issue as it complicates treatment strategies. This resistance is conferred by various types of beta-lactamases. Numerous studies report a high prevalence of ESBL-producing Gram-negative bacilli. The aim of this study was to identify the presence of the GES resistance gene in *E. coli* and *K. pneumoniae* strains in Burkina Faso.
Methods: In this study, 39 strains of E. coli and K. pneumoniae resistant to oxyimino-cephalosporins and monobactams were collected and analyzed for the presence of the blaGES beta-lactamase resistance gene using conventional PCR.
Results: Resistant strains were observed in 21 E. coli and 18 K. pneumoniae isolates. Among the ESBL-producing isolates, the GES gene was detected in 63% of E. coli and 37% of K. pneumoniae strains.
Conclusion: This study highlighted the presence of the GES gene in E. coli and K. pneumoniae strains resistant to oxyimino-cephalosporins and monobactams in Burkina Faso. This underscores the presence of novel ESBLs in the country, a finding of great importance for optimal patient care and antibiotic resistance control.

Mots-clés

Conventional PCR, GES gene, extended-spectrum beta-lactamases