Selection and validation of marker set for selection of resistant varieties of cowpea to Cowpea Aphid-borne Mosaic Virus (CABMV) in Burkina Faso

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Résumé

Objective: This study aims to validate of marker set for selection of resistant varieties of Cowpea Aphid-borneMosaic Virus. Methodology and results: A molecular characterization of five genotypes using seventeen (17) SSR markers was carried out. Conclusion and application of results: This study make it possible to identify four (4) polymorphic microsatellite markers (VM30, VM33, VM68 and VM70), that is to say a rate of 23, 52% of polymorphism versus 76, 47% of monomorphism. Two of the polymorphic markers- VM68 and VM30 were submitted to the test of validation. At the end of this test, VM68 was codominant, because it makes it possible to distinguish the heterozygous individuals (F 1 , BC 1 F 1 ) from the homozygous individuals (F 2 ) while the marker VM30 was dominating. The marker VM68 was validated and proposed in selection assisted by the markers of cowpea for resistance to CABMV. Keywords: cowpea, Cowpea Aphid-borne Mosaic Virus ((CABMV), Microsatellites, Validation, Burkina Faso

Mots-clés

Biology, Mosaic virus, Selection (genetic algorithm), Microsatellite, Marker-assisted selection, Genotype, Genetic marker, Veterinary medicine, Genetics, Plant virus, Virus, Gene, Medicine, Artificial intelligence